Weak laser effects on the biocompatibility of chitosan and Nafion as implantable glucose sensor outer materials

doi:10.3969/j.issn.2095-4344.0017

Abstract

Abstract:

BACKGROUND: The biocompatibility of chitosan and Nafion can be improved by external factors.

OBJECTIVE: To explore the effect of different weak laser irradiations (red, blue, green) on biocompatibility of porous chitosan membrane and the Nafion membrane.

METHODS: (1) Porous chitosan membrane test: Forty-eight Sprague-Dawley rats were randomized into red, green, blue light groups (n=16 per group). Porous chitosan membranes (two membranes at each side) were implanted into the bilateral subcutaneous tissue of the rat back with the spine as the axis of symmetry, and then the four implanted membranes in each rat were irradiated by red light for 0, 2, 4, 6 minutes respectively. The irradiation lasted until sample collection at 7, 14, 28 and 56 days after implantation, and the samples were used for histological analysis. The same procedures were done in the blue and green light groups. (2) Nafion membrane test: Twenty-four Sprague-Dawley rats were randomized into red, blue and green light groups (n=8 per group). Nafion membranes (two membranes at each side) were implanted into the bilateral subcutaneous tissue of the rat back with the spine as the axis of symmetry, and then the four implanted membranes in each rat were irradiated by red light for 0, 2, 4, 6 minutes respectively. The irradiation lasted until sample collection at 7 and 14 days after implantation, and the samples were used for histological analysis. The same procedures were done in the blue and green light groups.

RESULTS AND CONCLUSION: The content of red blood cells in blood vessels and vascular density around the membrane materials (porous chitosan membranes and Nafion membranes) increased after irradiated by red light (especially at 7 days after implantation); the red light had less influence on the inflammatory response and fibrous capsule thickness around the two kinds of membranes. The inflammatory cells percentage around the membrane materials irradiated by green light for 4 minutes was significantly reduced, and the blue light had less influence on inflammatory responses; blue and green lights showed effects on the fibrous capsule thickness and vascular density around the membrane materials, but the effect was not obvious. Thus, to a certain extent, weak lasers can improve the biocompatibility of PCSM and Nafion membrane.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Chitosan, Lasers, Biosensing Techniques, Tissue Engineering

CLC Number:

R318

Shen Hao, Liu Jun, Jing Wei-wei, Suo Yong-kuan, Chang Shi-jie, Sha Xian-zheng.

Weak laser effects on the biocompatibility of chitosan and Nafion as implantable glucose sensor outer materials

[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(2): 267-273.

Figures/Tables 7

2.4 蓝绿光照射多孔壳聚糖膜和Nafion膜3项观察指标统计分析实验对3组实验的形态学变化结果均统计了炎症细胞比、纤维包膜厚度及血管密度数据，图表结果均展示为统计学意义明显的数据，其他数据没有列出。蓝绿光照射多孔壳聚糖膜和Nafion膜周围炎性细胞面积比数据，见图6，7所示。多孔壳聚糖植入7 d，运用SPSS软件中单因素方差分析和LSD检测，绿光每天照射2，4 min的炎症细胞面积比小于照射0 min组(P=0.037，P=0.017)；对14 d的数据进行LSD检测，发现绿光2，4 min组的结果比照射 0 min小(P=0.176，P=0.048)，但没有明显差异；对于蓝光，各组之间的炎症细胞面积比没有明显差异。以上可看出，14 d的多孔壳聚糖膜周围炎症反应低于7 d的，蓝绿光照射7，14 d的结论相似，绿光能量在3.6 J/cm2(4 min)能较有效减轻炎症反应强度，而蓝光对多孔壳聚糖膜植入后的炎症影响较小。 Nafion膜植入7 d，运用SPSS软件中单因素方差分析和LSD检测，LSD检验显示照射各组与照射0 min组间差异显著(P=0.022，0.026，0.028)；而蓝光照射各组之间的差异无统计学意义。14 d的数据统计学分析表明，绿光各照射组值周围炎症反应小于照射0 min组(0.1<P <0.2)，但差异不显著，LSD检验显示各照射组之间的差异较小；蓝光各照射组和照射0 min组之间的结果较接近，差异小。以上可看出，Nafion膜的炎症反应比相同时期的多孔壳聚糖膜的弱，14 d的炎症反应比7 d弱，绿光各照射组都能减弱Nafion膜植入短期内的炎症反应，而蓝光没有这种效果。蓝绿光对多孔壳聚糖膜和Nafion膜周围纤维包膜厚度和血管密度影响较小，而且变化无明显规律。整体上，绿光在一定程度能提高膜材料的生物相容性。"

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